The Patterns of Restriction Fragment of Several Enzymes to Distinguish Toxoplasma gondii Isolates Virulent and Avirulent Strains using GRA1 and GRA7 Genetic Marker

Toxoplasma gondii pathogenicity depends on the type derived from a clonal population. A genetic analysis of locus has been carried out to determine different genotypes T. (strain types I, II, and III) that are associated with human toxoplasmosis. The several linked toxoplasmosis have identified through study locus. In this investigation, PCR-RFLP was found be useful, simple method genotypic characterization. objective genotyping characterize RH BEV strains isolates by using restriction enzymes. tachyzoite DNA extracted amplified PCR dense granule markers (GRA1 GRA7) designed Primer3plus. amplification were digested products used classify into two (virulent avirulent). results demonstrated RFLP patterns GRA1 GRA7 gene area DdeI, MvaI, HinfI, RsaI, Sau96I enzymes can identify virulent or avirulent gondii. strain produced digestion product which distinguished strains.